Coding

Part:BBa_K3117023:Design

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-13)


scFv VL against GPA33 codon optimized for CHO expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was codon optimized for expression in mammalian cells.


Source

The sequence of this part origins from the patent AU2018260975A1 [2].

References

[1]: Carreras-Sangrà, Nelson; Tomé-Amat, Jaime; García-Ortega, Lucía; Batt, Carl A.; Oñaderra, Mercedes; Martínez-del-Pozo, Alvaro et al. (2012): Production and characterization of a colon cancer-specific immunotoxin based on the fungal ribotoxin α-sarcin. In: Protein engineering, design & selection : PEDS 25 (8), S. 425–435. DOI: 10.1093/protein/gzs032.

[2]: Bonvini, E., Moore, P. A., Li, J. C., Johnson, L. S., & Shah, K. (2017). U.S. Patent Application No. 15/313,765.